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The video mentions that you need to pay attention to the sign of the strain value only, not the direction of the coordinate system. For example,  you should not be concerned, if say, the x-direction for one of your gauge points in the opposite direction of the other two gauges in the rosette. This is because the strain transformation is invariant when you rotate a coordinate direction by 180 deg. The transformation formula, , has cos(2*theta) and sin(2*theta). When theta = 180 deg, cos(2*theta) and sin(2*theta) will be the same as when theta = 0.