Name Size Creator Creation Date Labels Comment  
PDF File PCR Product Purification and Sanger Sequencing Submission_10112019.pdf 344 kB Samuel Joseph Reichler May 18, 2020 15:06
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Updated to current SOP format
PDF File 8.1.1.1.18-Gel Electrophoresis of PCR Product.pdf 371 kB Samuel Joseph Reichler May 18, 2020 15:05
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Microsoft Word Document pGEM Ladder Recipe.docx 95 kB Samuel Joseph Reichler May 18, 2020 15:02
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Converted to SOP format
PDF File 8.4.5 Creating Consensus DNA-Sequencher.pdf 723 kB Samuel Joseph Reichler May 04, 2020 10:38
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PDF File 8.1.1.1.20-rpoB_ATassignment.pdf 892 kB Samuel Joseph Reichler May 04, 2020 10:34
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PDF File 8.1.1.3.6-InvA colony PCR for Salm.pdf 142 kB Samuel Joseph Reichler May 04, 2020 10:24
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Made to conform with new SOP format.
PDF File 8.1.1.4.5.1-PCR Amplification of 16s rDNA Internal Region.pdf 756 kB user-54a31 Jan 30, 2020 17:21
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added instructions to make dNTPs and primers, and final reaction concentrations
PDF File 8.3.14_HighThroughput_gDNA_Extraction_Qiagen.pdf 477 kB user-54a31 Jan 16, 2020 13:46
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PDF File Gel Electrophoresis of PCR Product_10112019.pdf 176 kB Samuel Joseph Reichler Oct 24, 2019 16:17
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PDF File Automated Sequence Editing SOP.pdf 905 kB Samuel Joseph Reichler Oct 24, 2019 16:14
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Updated to current SOP format
PDF File 8.8.14_RNAextSalmonella.pdf 147 kB Samuel Joseph Reichler Mar 14, 2019 17:10
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PDF File P22 Transduction of Salmonella_12JUL17.pdf 114 kB Samuel Joseph Reichler Jul 28, 2017 09:00
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PDF File Assembly_submission_NCBI_WGS.pdf 472 kB Samuel Joseph Reichler Dec 23, 2016 13:53
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PDF File Short_read_submission_SRA_SOP.pdf 368 kB Samuel Joseph Reichler Dec 23, 2016 13:53
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PDF File Quality_control_and_assembly_SOP.pdf 668 kB Samuel Joseph Reichler Dec 23, 2016 13:52
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PDF File SOP for amplifying full length and internal region of 16s.pdf 284 kB Samuel Joseph Reichler Nov 18, 2016 16:05
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Changed reference to 16s internal fragment PCR to refer to new SOP on wiki.
PDF File Primer Checklist -VGO 10-31-16.pdf 263 kB Samuel Joseph Reichler Nov 02, 2016 12:05
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Microsoft Excel Spreadsheet Example Nextera Library Sample Preparation Spreadsheet.xlsx 37 kB Samuel Joseph Reichler Nov 02, 2016 11:56
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PDF File CDC MiSeq Nextera XT Library Preparation Protocol.pdf 653 kB Samuel Joseph Reichler Nov 02, 2016 11:53
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PDF File 8.3.16-WGS_SOP_June2016.pdf 999 kB Samuel Joseph Reichler Nov 02, 2016 11:45
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SOP updated by Sophia to include instructions for Qubit usage both at the BRC and in the Dando lab.
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