8.1- Characterization & DNA amplification
8.1.1-PCR
8.1.1.1-General PCR Methods & Guidelines
8.1.1.1.1-16S rDNA PCR-Go Taq.pdf
8.1.1.1.2-16S rDNA PCR-modified for Taq gold.pdf
8.1.1.1.3-Basic GoTaq PCR Protocol.pdf
8.1.1.1.4-Colony PCR Protocol.pdf
8.1.1.1.5-Guidelines for Primer Design.pdf
8.1.1.1.8-Sample Submission to BRC.pdf
8.1.1.1.9-Ten Things That Can Kill Your PCR.pdf
8.1.1.1.10-Submitting sequences to GenBank.pdf
8.1.1.1.11-Primer Database Search Protocol.pdf
8.1.1.1.12-rpoB_sigB_ATassignment_User.pdf
8.1.1.2-Listeria PCR
8.1.1.2.1-Ten Gene MLST for Listeria
8.1.1.2.1.1-10 gene MLST scheme.xls
8.1.1.2.1.2-addB PCR-GoTaq.xls
8.1.1.2.1.3-ldh PCR-GoTaq.xls
8.1.1.2.1.4-lmo0490 PCR-GoTaqhotstart.xls
8.1.1.2.1.5-lmo1555 PCR-GoTaq.xls
8.1.1.2.1.6-lmo2763 PCR-GoTaqhotstart.xls
8.1.1.2.1.7-pbpA PCR-GoTaqhotstart.xls
8.1.1.2.1.8-pbpA PCR-J1-208 GoTaqhotstart.xls
8.1.1.2.1.9-polC PCR-GoTaq.xls
8.1.1.2.1.10-prs PCR-GoTaq.xls
8.1.1.2.1.11-rarA PCR-GoTaq.xls
8.1.1.2.2-Listeria PCRs by Gene Name
8.1.1.2.2.1-Fact Sheet on ActA Differentiation PCR and Interpretation of Results.pdf
8.1.1.2.2.2-Gap PCR protocol for Listeria monocytogenes.pdf
8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenes.2013.pdf
8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and Sequencing.pdf
8.1.1.2.2.6-Multiplex PCR for inlH screening.pdf
8.1.1.2.2.7-Nested hly PCR for Listeria monocytogenes.pdf
8.1.1.2.2.8-Protocol for sequencing actA gene most polymorphic region.pdf
8.1.1.2.2.9-Protocol for Sequencing actA gene region with best discrimination.pdf
8.1.1.2.2.10-Protocol for Sequencing inlA gene region with the best discrimination.pdf
8.1.1.2.2.11-Prs PCR Protocol for Listeria monocytogenes.pdf
8.1.1.2.2.12-PurM PCR Protocol for lineage II Listeria monocytogenes.pdf
8.1.1.2.2.13-PurM PCR Protocol for Listeria monocytogenes.pdf
8.1.1.2.2.14-RibC PCR Protocol for Listeria monocytogenes.pdf
8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdf
8.1.1.3-Salmonella PCR
8.1.1.3.1-fimA PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf
8.1.1.3.2-manB PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf
8.1.1.3.3-mdh PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf
8.1.1.3.4-7 Gene MLST for Salmonella.pdf
8.1.1.3.4.1-7 gene MLST for Salmonella- Primers and PCR.xls
8.1.1.3.4.2-Salmonella 7 gene MLST Template.xls
8.1.1.3.5-PCR detection of the presence or absence of antibiotic resistance genes in Salmonella.pdf
8.1.1.3.6-InvA colony PCR for Salm 4-13-10.pdf
8.1.1.3.7-PCR identification of common Salmonella serogroups.pdf
8.1.1.3.8-PCR determination of H1 and H2-antigens for Salmonella.pdf
8.1.1.4-PCRs for Other Organisms
8.1.1.4.1-E. coli 6-gene multiplex PCR.pdf
8.1.1.4.2-ipaH colony PCR for Shigella.pdf
8.1.1.4.3-SodA PCR Protocol for S. agalactiae.pdf
8.1.2-Phenotypic Characterization
8.1.2.1-Semi-Quantitative Hemolysis Assay for Listeria.pdf
8.1.3-RLFP
8.1.3.1-Polymerase Chain Reaction- Restriction Fragment Length Polymorphism.pdf
8.1.4-Southern Blot
8.1.4.1-Southern Blotting by Electrophoretic Transfer and non radioactive Detection.pdf
8.1.4.3-SouthernBlot Hybridization.pdf
8.2-DNA Labeling & Modification
8.2.1-3’ end labeling of oligonucleotides.pdf
8.2.2-Kinasing and DIG labeling of LCR primer.pdf
8.2.3-Poly-T tailing of oligonucleotides.pdf
8.2.4-Protocol for Fluorescein labeling of oligonucleotides carrying an aminolink.pdf
8.2.5-Protocol for Geneclean (USB).pdf
8.3-DNA preparation, Purification, & Quantification
8.3.1-Chelex DNA extraction from blood.pdf
8.3.2-Chelex DNA extraction from semen.pdf
8.3.3-Phenol Chloroform Listeria gDNA Extraction_1_28_2015.pdf
8.3.4-DNA Preparation from Listeria using Guanidinium Isothiocyanate.pdf
8.3.5-Listeria Lysis Methods and Procedures_11_26_13.pdf
8.3.6-PCR product purification using ExoSAP.pdf
8.3.7-PCR Purification Protocol for DNA Sequencing.pdf
8.3.10-Quantification of Purified DNA by NanoDrop.pdf
8.3.11-Single Stranded DNA preparation from M13.pdf
8.3.12-Small-scale phenolchloroform extraction of chromosomal DNA from Listeria monocytogenes.pdf
8.3.13_Genomic_DNA_Extraction_Qiagen_11_21_13.pdf
8.3.14_HighThroughput_gDNA_Extraction_Qiagen_1_28_15.pdf
8.3.15_Take3_NucleicAcid_Quantification_01_20_15.pdf
8.4-DNA Recombination & Mutagenesis
8.4.1-Cloning of DNA Flanking Tn917-LTV3.pdf
8.4.2-Cloning of DNA Fragments.pdf
8.4.3-Cloning PCR products for Sequencing using pCR 2.1.pdf
8.4.4-Cloning Protocol for Transformation of E. coli DH5aF.pdf
8.4.5-Electroporation L mono.pdf
8.4.6-Electroporation of E coli.pdf
8.4.7-Electroporation of Mycobacterium paratuberculosis.pdf
8.4.9-GUS promoter fusions in Listeria monocytogenes.pdf
8.4.10-Integration of pPL2 derivatives into L. monocytogenes 10403S.pdf
8.4.11-Listeria Genomic Alterations.pdf
8.4.12-Overview-Making a mutant in Listeria monocytogenes.pdf
8.4.13-Part II-Making a mutant in Listeria monocytogenes.pdf
8.4.14-Part III-Making a mutant in Listeria monocytogenes.pdf
8.4.15-Part IV-Making a mutant in Listeria monocytogenes.pdf
8.4.19-Procedure for cloning with pDH32.pdf
8.4.20-Whole Mutant- SoeingPCR for L. mono.pdf
8.5-Microarray
8.5.1-Analyzing Microarrays.pdf
8.5.2-Creating Microarrays.pdf
8.5.3-Labeling RNA with Cy3Cy5 and Hybridization for Microarrays.pdf
8.5.4-Microarray Slide Blocking.pdf
8.6-Protein Methods
8.6.2-Beta Galactosidase Assay.pdf
8.6.3-His tagged sigma factor protein purification protocol.pdf
8.7-RNA Prep & Analysis
8.7.1-DNase treatment and phenolchloroform extractions for high purity RNA.pdf
8.7.2-Isolation of RNA from Listeria monocytogenes infected mammalian cells.pdf
8.7.3-Preparation of infected tissue culture cells for bacterial RNA collection.pdf
8.7.4-Preperation of Listeria RNA.pdf
8.7.5-RNA extraction, clean-up and storage using Turbo DNase for TaqMan.pdf
8.7.6-RNA Prep with RNeasy Midi Kit instructions.pdf
8.7.7-RNeasy kit total RNA isolation from Listeria monocytogenes with on-column DNase treatment.pdf
8.7.8-RT-PCR for the detection of cytokine mRNA.pdf