8.1- Characterization & DNA amplification
8.1.1-PCR
8.1.1.1-General PCR Methods & Guidelines
8.1.1.1.1-16S rDNA PCR-Go Taq.pdf
8.1.1.1.2-16S rDNA PCR-modified for Taq gold.pdf
8.1.1.1.3-Basic GoTaq PCR Protocol.pdf
8.1.1.1.4-Colony PCR Protocol.pdf
8.1.1.1.5-Guidelines for Primer Design.pdf
8.1.1.1.6-Primer Naming, Reconstitution and Storage Protocol.pdf
8.1.1.1.7-Primer Reconstitution.pdf
8.1.1.1.8-Sample Submission to BRC.pdf
8.1.1.1.9-Ten Things That Can Kill Your PCR.pdf
8.1.1.1.10-Submitting sequences to GenBank.pdf
8.1.1.2-Listeria PCR
8.1.1.2.1-Ten Gene MLST for Listeria
8.1.1.2.1.1-10 gene MLST scheme.xls
8.1.1.2.1.2-addB PCR-GoTaq.xls
8.1.1.2.1.3-ldh PCR-GoTaq.xls
8.1.1.2.1.4-lmo0490 PCR-GoTaqhotstart.xls
8.1.1.2.1.5-lmo1555 PCR-GoTaq.xls
8.1.1.2.1.6-lmo2763 PCR-GoTaqhotstart.xls
8.1.1.2.1.7-pbpA PCR-GoTaqhotstart.xls
8.1.1.2.1.8-pbpA PCR-J1-208 GoTaqhotstart.xls
8.1.1.2.1.9-polC PCR-GoTaq.xls
8.1.1.2.1.10-prs PCR-GoTaq.xls
8.1.1.2.1.11-rarA PCR-GoTaq.xls
8.1.1.2.2-Listeria PCRs by Gene Name
8.1.1.2.2.1-Fact Sheet on ActA Differentiation PCR and Interpretation of Results.pdf
8.1.1.2.2.2-Gap PCR protocol for Listeria monocytogenes.pdf
8.1.1.2.2.3-Hly ab gene PCR for Listeria monocytogenes.pdf
8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and Sequencing.pdf
8.1.1.2.2.5-Modified SigB PCR Protocol-Taq Gold 10uM primers.pdf
8.1.1.2.2.6-Multiplex PCR for inlH screening.pdf
8.1.1.2.2.7-Nested hly PCR for Listeria monocytogenes.pdf
8.1.1.2.2.8-Protocol for sequencing actA gene most polymorphic region.pdf
8.1.1.2.2.9-Protocol for Sequencing actA gene region with best discrimination.pdf
8.1.1.2.2.10-Protocol for Sequencing inlA gene region with the best discrimination.pdf
8.1.1.2.2.11-Prs PCR Protocol for Listeria monocytogenes.pdf
8.1.1.2.2.12-PurM PCR Protocol for lineage II Listeria monocytogenes.pdf
8.1.1.2.2.13-PurM PCR Protocol for Listeria monocytogenes.pdf
8.1.1.2.2.14-RibC PCR Protocol for Listeria monocytogenes.pdf
8.1.1.2.2.15-SigB PCR Protocol GoTaq.pdf
8.1.1.3-Salmonella PCR
8.1.1.3.1-fimA PCR and DNA sequencing of Salmonella enterica.pdf
8.1.1.3.2-manB PCR and DNA sequencing of Salmonella enterica.pdf
8.1.1.3.3-mdh PCR and DNA sequencing of Salmonella enterica.pdf
8.1.1.3.4-7 Gene MLST for Salmonella.pdf
8.1.1.3.4.1-7 gene MLST for Salmonella- Primers and PCR.xls
8.1.1.3.4.2-Salmonella 7 gene MLST Template.xls
8.1.1.3.5-PCR detection of the presence or absence of antibiotic resistance genes in Salmonella.pdf
8.1.1.3.6-InvA colony PCR for Salm 4-13-10.pdf
8.1.1.3.7-PCR identification of common Salmonella serogroups.pdf
8.1.1.3.8-PCR determination of H1 and H2-antigens for Salmonella.pdf
8.1.1.4-PCRs for Other Organisms
8.1.1.4.1-E. coli 6-gene multiplex PCR.pdf
8.1.1.4.2-ipaH colony PCR for Shigella.pdf
8.1.1.4.3-SodA PCR Protocol for S. agalactiae.pdf
8.1.2-Phenotypic Characterization
8.1.2.1-Restriction Digest and Cloning of PCR amplified 16S rDNA.pdf
8.1.2.2-Semi-Quantitative Hemolysis Assay for Listeria.pdf
8.1.3-RLFP
8.1.3.1-Polymerase Chain Reaction- Restriction Fragment Length Polymorphism.pdf
8.1.4-Southern Blot
8.1.4.1-Southern Blotting by Electrophoretic Transfer and non radioactive Detection.pdf
8.1.4.3-SouthernBlot Hybridization.pdf
8.2-DNA Labeling & Modification
8.2.1-3' end labeling of oligonucleotides.pdf
8.2.2-Kinasing and DIG labeling of LCR primer.pdf
8.2.3-Poly-T tailing of oligonucleotides.pdf
8.2.4-Protocol for Fluorescein labeling of oligonucleotides carrying an aminolink.pdf
8.2.5-Protocol for Geneclean (USB).pdf
8.3-DNA preparation
8.4-DNA recombination & mutagenesis
8.5-Microarray
8.6-Protein Methods
8.7-RNA Prep & Analysis