You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 21 Next »

Characterization & DNA amplification

General PCR Methods & Guidelines

8.1.1.1.1-16S rDNA PCR-Go Taq.pdf

8.1.1.1.2-16S rDNA PCR-modified for Taq gold.pdf

8.1.1.1.3-Basic GoTaq PCR Protocol.pdf

8.1.1.1.4-Colony PCR Protocol.pdf

8.1.1.1.5-Guidelines for Primer Design.pdf

8.1.1.1.6-Primer Naming, Reconstitution and Storage Protocol.pdf

8.1.1.1.7-Primer Reconstitution.pdf

8.1.1.1.8-Sample Submission to BRC.pdf

8.1.1.1.9-Ten Things That Can Kill Your PCR.pdf

8.1.1.1.10-Submitting sequences to GenBank.pdf

Listeria PCR

8.1.1.2.1.1-10 gene MLST scheme.xls

8.1.1.2.1.2-addB PCR-GoTaq.xls

8.1.1.2.1.3-ldh PCR-GoTaq.xls

8.1.1.2.1.4-lmo0490 PCR-GoTaqhotstart.xls

8.1.1.2.1.5-lmo1555 PCR-GoTaq.xls

8.1.1.2.1.6-lmo2763 PCR-GoTaqhotstart.xls

8.1.1.2.1.7-pbpA PCR-GoTaqhotstart.xls

8.1.1.2.1.8-pbpA PCR-J1-208 GoTaqhotstart.xls

8.1.1.2.1.9-polC PCR-GoTaq.xls

8.1.1.2.1.10-prs PCR-GoTaq.xls

8.1.1.2.1.11-rarA PCR-GoTaq.xls

8.1.1.2.2.1-Fact Sheet on ActA Differentiation PCR and Interpretation of Results.pdf

8.1.1.2.2.2-Gap PCR protocol for Listeria monocytogenes.pdf

8.1.1.2.2.3-Hly ab gene PCR for Listeria monocytogenes.pdf

8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and Sequencing.pdf

8.1.1.2.2.5-Modified SigB PCR Protocol-Taq Gold 10uM primers.pdf

8.1.1.2.2.6-Multiplex PCR for inlH screening.pdf

8.1.1.2.2.7-Nested hly PCR for Listeria monocytogenes.pdf

8.1.1.2.2.8-Protocol for sequencing actA gene most polymorphic region.pdf

8.1.1.2.2.9-Protocol for Sequencing actA gene region with best discrimination.pdf

8.1.1.2.2.10-Protocol for Sequencing inlA gene region with the best discrimination.pdf

8.1.1.2.2.11-Prs PCR Protocol for Listeria monocytogenes.pdf

8.1.1.2.2.12-PurM PCR Protocol for lineage II Listeria monocytogenes.pdf

8.1.1.2.2.13-PurM PCR Protocol for Listeria monocytogenes.pdf

8.1.1.2.2.14-RibC PCR Protocol for Listeria monocytogenes.pdf

8.1.1.2.2.15-SigB PCR Protocol GoTaq.pdf

Salmonella PCR

8.1.1.3.1-fimA PCR and DNA sequencing of Salmonella enterica.pdf

8.1.1.3.2-manB PCR and DNA sequencing of Salmonella enterica.pdf

8.1.1.3.3-mdh PCR and DNA sequencing of Salmonella enterica.pdf

8.1.1.3.4-7 Gene MLST for Salmonella.pdf

8.1.1.3.4.1-7 gene MLST for Salmonella- Primers and PCR.xls

8.1.1.3.4.2-Salmonella 7 gene MLST Template.xls

8.1.1.3.5-PCR detection of the presence or absence of antibiotic resistance genes in Salmonella.pdf

8.1.1.3.6-InvA colony PCR for Salm 4-13-10.pdf

PCRs for Other Organisms

8.1.1.4.1-E. coli 6-gene multiplex PCR.pdf

8.1.1.4.2-ipaH colony PCR for Shigella.pdf

8.1.1.4.3-SodA PCR Protocol for S. agalactiae.pdf

Phenotypic Characterization

8.1.2.1-Restriction Digest and Cloning of PCR amplified 16S rDNA.pdf

8.1.2.2-Semi-Quantitative Hemolysis Assay for Listeria.pdf

RLFP

8.1.3.1-Polymerase Chain Reaction- Restriction Fragment Length Polymorphism.pdf

Southern Blot

8.1.4.1-Southern Blotting by Electrophoretic Transfer and non radioactive Detection.pdf

8.1.4.2-Southern Blotting.pdf

8.1.4.3-SouthernBlot Hybridization.pdf

DNA Labeling & Modification

DNA preparation

DNA recombination & mutagenesis

Microarray

Protein Methods

RNA Prep & Analysis

  • No labels