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8.1- Characterization & DNA amplification

8.1.1-PCR

8.1.1.1-General PCR Methods & Guidelines

8.1.1.1.1-16S rDNA PCR-Go Taq.pdf

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8.1.1.1.10-Submitting sequences to GenBank.pdf

8.1.1.2-Listeria PCR

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8.1.1.2.1-Ten Gene MLST for Listeria

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8.1.1.2.1.1-10 gene MLST scheme.xls

8.1.1.2.1.2-addB PCR-GoTaq.xls

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8.1.1.2.1.11-rarA PCR-GoTaq.xls

8.1.1.2.2-Listeria PCRs by Gene Name

8.1.1.2.2.1-Fact Sheet on ActA Differentiation PCR and Interpretation of Results.pdf

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8.1.1.2.2.15-SigB PCR Protocol GoTaq.pdf

8.1.1.3-Salmonella PCR

8.1.1.3.1-fimA PCR and DNA sequencing of Salmonella enterica.pdf

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8.1.1.3.8-PCR determination of H1 and H2-antigens for Salmonella.pdf

8.1.1.4-PCRs for Other Organisms

8.1.1.4.1-E. coli 6-gene multiplex PCR.pdf

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8.1.1.4.3-SodA PCR Protocol for S. agalactiae.pdf

8.1.2-Phenotypic Characterization

8.1.2.1-Restriction Digest and Cloning of PCR amplified 16S rDNA.pdf

8.1.2.2-Semi-Quantitative Hemolysis Assay for Listeria.pdf

8.1.3-RLFP

8.1.3.1-Polymerase Chain Reaction- Restriction Fragment Length Polymorphism.pdf

8.1.4-Southern Blot

8.1.4.1-Southern Blotting by Electrophoretic Transfer and non radioactive Detection.pdf

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8.1.4.3-SouthernBlot Hybridization.pdf

8.2-DNA Labeling & Modification

8.2.1-3' end labeling of oligonucleotides.pdf

8.2.2-Kinasing and DIG labeling of LCR primer.pdf

8.2.3-Poly-T tailing of oligonucleotides.pdf

8.2.4-Protocol for Fluorescein labeling of oligonucleotides carrying an aminolink.pdf

8.2.5-Protocol for Geneclean (USB).pdf

8.3-DNA preparation

8.4-DNA recombination & mutagenesis

8.5-Microarray

8.6-Protein Methods

8.7-RNA Prep & Analysis