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When you make a connection utilizing a push-to-connect connector, ensure that you push the tube as fully in as you can go. You should feel a pop, when the tube is fully in and is caught by the push-to-connect connector.
Sample Experimental Set-up
With rubber tubing, ensure that the tubing tightly fits over the barbed part.
Exercise in Experimental Design and Set-Up
Here is a sample exercise that should help to set up an experiment in lab. In a group or individually sketch out an experimental schematic of a mixing process to blend to chemicals.
On one end you want to have a chemical stock tank in a one liter bottle hooked up to a computer controlled peristaltic pump. The pump will meter in the chemical at a set rate to a 4 L tank that has a pressure sensor set up at the bottom to record the height of water. For part of the experiment you want to meter in the chemical, however for another part of the experiment you do not want to meter in the chemical.
In your drawing, you can indicate a peristaltic pump as a circle inside of a square, a chemical stock tank as a bottle, a solenoid valve with an X and tubing as a straight line.
Click here to see how closely your schematic matches this one
In the drawing label important experimental parameters such as concentration of chemical stock, flowrate of chemical stock.