The following protocols are no longer used or updated by our labs. Please contact Martin Wiedmann, Nicole Martin, Ahmed Gaballa, Sam Reichler, or Jordan Skeens to request access to these these protocols.
7.1-2nd floor-16S GreenMaster Procedure.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.2-16s Identification Using RDP database.pdf [archived 10April2020]
7.3-96 Well Plate Procedure.pdf Archived protocol (created 2006) [archived March 2016]; describes procedure for submitting 96 well plate with purified PCR products for sequencing
7.3.1-96WellPlate Template.xls [archived 10April2020]
7.5-Colony PCR.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.7-Endospore Stain - cold.pdf [archived 10April2020]
7.8-Endospore Staining -- Steam Method.pdf [archived 10April2020]
7.9-Final rpoB GreenMaster Protocol.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.10-Growth Rate L.mono in Milk.pdf [archived 10April2020]
7.12-Identification of rpoB Sequences using Bioedit.pdf [archived 10April2020]
7.14-PCR Procedure and Touble-shooting Guide for rpoB and 16S.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.15-Primer Ordering.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.16-Primer Reconstitution.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.17-Procedure for Lysis of Bacterial Cells.pdf Archived Protocol [archived 2019-10-30 by sjr267]
7.18-Purified Lysates Procedure.pdf