8.1.1.1.5-Guidelines for Primer Design [Updated by vg93 27Oct2016]
8.1.1.1.5.1-Primer Design Checklist [created by vg93 31Oct2016]
8.1.1.1.7-Primer Ordering and Reconstitution SOP [updated 2020-06-01 ram524]
8.1.1.1.9-Ten Things That Can Kill Your PCR Information Sheet [reviewed by mw16 07072016]
8.1.1.1.10-Submitting sequences to GenBank SOP [reviewed by mw16 07072016]
8.1.1.1.11-Primer Database Search Protocol.pdf SOP [updated 2020-06-01 ram524]
8.1.1.1.12-rpoB sigB AT Assignment [MW]
8.1.1.1.17-pGEM Ladder for Size Determination of PCR Products SOP [updated by ser15 2020-05-18]
8.1.1.1.18-Gel Electrophoresis of PCR Product SOP [updated 2020-05-18 by ser15]
8.1.1.1.19-PCR Product Purification and Sanger Sequencing Submission SOP [updated 2020-05-18 by ser15]
8.1.1.2.2-Listeria PCRs by Gene Name
8.1.1.2.2.1-actA PCR SOP [updated 2020-05-18 by ser15]
8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenes SOP [updated 2020-05-18 by ser15]
8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and Sequencing SOP [reviewed by mw16 07072016]
8.1.1.2.2.5-sigB PCR SOP [updated 2020-01-30 by js2568]
8.1.1.2.2.17-Degenerate sigB PCR SOP [updated 2020-09-05 by ser15]
8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdf (Yichang and Veronica)
8.1.1.3.6-InvA colony PCR for Salmonella SOP [updated 2020-04-27 amb629]
8.1.1.3.7-PCR identification of common Salmonella serogroups SOP [reviewed by mw16 07072016]
8.1.1.3.8-PCR determination of H1 and H2-antigens for Salmonella SOP [reviewed by mw16 07072016]
8.1.1.4.1-E. coli 6-gene multiplex PCR.pdf
8.1.1.4.4-rpoB PCR Protocol SOP [updated 2020-05-18 by ser15]
8.1.1.4.5-PCR Amplification of Full Length 16s rDNA SOP [updated 18Nov2016 by sjr267]
8.1.1.4.5.1-PCR Amplification of 16s rDNA Internal Region SOP [updated 2020-01-30 by js2568]
8.1.1.4.6-ITS PCR for Yeast and Mold SOP [updated 2020-06-26 by rle58]
[see archive]
[see archive]
[see archive]
8.3.10-Quantification of Purified DNA by NanoDrop.pdf
8.3.13-Genomic_DNA_Extraction_Qiagen SOP [updated by ajh22 4/28/16]
8.3.14-HighThroughput_gDNA_Extraction_Qiagen_1_28_15.pdf
8.3.15-Take3_NucleicAcid_96_well_Quantification_01_20_15 Protocol [reviewed by mw16 07072016]
8.3.16-DNA Preparation for Whole Genome Sequencing SOP [updated 27Oct2016 by sh988]
8.3.16.1-Step-by-step checklist for Gram+ bacteria Supplemental file [effective 2Sept2016 ajh22]
8.3.16.2-Step-by-step checklist for Gram- bacteria Supplemental file [effective 13Sept2016 ajh22]
8.3.16.3-CDC MiSeq Nextera XT Library Preparation Reference [reviewed 07Oct2016 by jk2739]
8.3.16.4-Example Nextera Library Sample Preparation Spreadsheet Template [reviewed 07Oct2016 by jk2739]
8.3.18-Preparing Cell Lysates for PCR SOP [updated 2020-05-18 by ser15]
Sanger Sequencing
8.4.1-Automated Sequence Editing SOP [updated 2019-10-11 by js2568]
8.4.5-Creating Consensus DNA Sequences using Sequencher SOP [created 2020-04-28 ag67]
8.4.6-rpoB Allelic Type (AT) Assignment SOP [updated 2020-04-28 ag67]
Whole Genome Sequencing
8.4.2-Quality Control and Assembly SOP [reviewed by jk2739 12/19/2016]
8.4.3-Short Read Submission (SRA) SOP [reviewed by jk2739 12/19/2016]
8.4.4-Assembly submission to NCBI SOP [reviewed by rho2 11/25/2016]
8.5.2-Cloning of DNA Fragments Protocol [reviewed 8Aug2016 mw16]
8.5.9-GUS promoter fusions in Listeria monocytogenes Protocol [reviewed 08Aug2016 mw16]
8.5.10-Integration of pPL2 derivatives into L. monocytogenes 10403S Protocol [reviewed 08Aug2016 mw16]
8.5.16-pMJG2 vector map.jpg Plasmid Map [reviewed 08Aug2016 mw16]
8.5.17-pMJG3 vector map.jpg Plasmid Map [reviewed 08Aug2016 mw16]
8.5.18-pMJG4 vector map.jpg Plasmid Map [reviewed 08Aug2016 mw16]
8.5.20-Whole Mutant-Soeing PCR for L. mono SOP [reviewed by bmb29 4/6/2016]
8.5.21-P22 Transduction of Salmonella Protocol [reviewed by ram524 28Jul2017]
8.5.22-Protocol for Lambda Red Recombinase in Salmonella SOP [updated by ram524 2020-06-01]
8.5.23-Gene Deletion Using I-SceI coupled with Lambda Red Recombination in Salmonella SOP [created 2020-06-01 by ram524]
[see archive]
8.7.1-Assay for beta-galactosidase activity of B. subtilis transformed with lacZ reporter plasmid Protocol [mw16 checked07072016]
8.7.2-Beta Galactosidase Assay_general Protocol [mw16 checked07072016]
8.7.3-His tagged sigma factor protein purification protocol Protocol [mw16 checked07072016]
8.8.11-RNA Extraction Using TriReagent SOP [updated by arc326 2020-05-18]
8.8.13-Sybr Green qRT-PCR Protocol [updated by arc326 2020-05-18]
8.8.14-RNA Extraction from Salmonella using Tri-Reagent SOP [updated by arc326 2020-05-18]
8.8.15-RNA extraction from Salmonella using RNeasy Kit SOP [updated 2020-06-01 by ram524]