8.1- Characterization & DNA amplification
8.1.1-PCR
8.1.1.1-General PCR Methods & Guidelines
8.1.1.1.5-Guidelines for Primer Design [Updated by vg93 27Oct2016]
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8.1.1.2-Listeria PCR
8.1.1.2.1-Ten Gene MLST for Listeria
8.1.1.2.1.1-10 gene MLST scheme.xls [reviewed by mw16 07072016]
8.1.1.2.1.2-addB PCR-GoTaq.xls [reviewed by mw16 07072016]
8.1.1.2.1.3-ldh PCR-GoTaq.xls [reviewed by mw16 07072016]
8.1.1.2.1.4-lmo0490 PCR-GoTaqhotstart.xls [reviewed by mw16 07072016]
8.1.1.2.1.5-lmo1555 PCR-GoTaq.xls [reviewed by mw16 07072016]
8.1.1.2.1.6-lmo2763 PCR-GoTaqhotstart.xls [reviewed by mw16 07072016]
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8.1.1.2.1.8-pbpA PCR-J1-208 GoTaqhotstart.xls [reviewed by mw16 07072016]
8.1.1.2.1.9-polC PCR-GoTaq.xls [reviewed by mw16 07072016]
8.1.1.2.1.10-prs PCR-GoTaq.xls [reviewed by mw16 07072016]
8.1.1.2.1.11-rarA PCR-GoTaq.xls [reviewed by mw16 07072016]
8.1.1.2.2-Listeria PCRs by Gene Name
8.1.1.2.2.1-actA PCR SOP [updated 2020-01-30 by js2568]
8.1.1.2.2.2-Gap PCR protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
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8.1.1.2.2.5-sigB PCR SOP [updated 2020-01-30 by js2568]
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8.1.1.2.2.12-purM PCR Protocol for lineage II Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.13-purM PCR Protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.14-ribC PCR Protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdf (Yichang and Veronica)
8.1.1.3-Salmonella PCR
8.1.1.3.4-7 Gene MLST for Salmonella Informational Document [reviewed by mw16 07072016]
8.1.1.3.4.1-7 gene MLST for Salmonella- Primers and PCR.xls (check)
8.1.1.3.4.2-Salmonella 7 gene MLST Template.xls (check)
8.1.1.3.6-InvA colony PCR for Salmonella PCR Protocol [updated 09Aug2016 ajh22]
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8.1.1.4.1-E. coli 6-gene multiplex PCR.pdf8.1.1.4.2-ipaH colony PCR for Shigella.pdf
8.1.1.4.3-SodA PCR Protocol for S. agalactiae.pdf8.1.1.4.4-rpoB PCR Protocol SOP [updated 2019-12-20 by js2568]
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8.1.2-Phenotypic Characterization
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[see archive below]
8.1.3-RFLP
[see archive below]
8.1.4-Southern Blot
[archive whole section]
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[see archive
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8.3-DNA preparation, Purification, & Quantification
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8.6-Microarray
[see archive below]
8.7-Protein Methods
8.7.1-Assay for beta-galactosidase activity of B. subtilis transformed with lacZ reporter plasmid Protocol [mw16 checked07072016]
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