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8.1.1.1-General PCR Methods & Guidelines
8.1.1.1.3-Basic GoTaq PCR Protocol.pdf [check with current users; keep]
8.1.1.1.4-Colony PCR Protocol [updated 29Jul2016 jit7]
8.1.1.1.5-Guidelines for Primer Design [Updated by vg93 27Oct2016]
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8.1.1.1.7-Primer Ordering and Reconstitution SOP [updated 2019-03-14 ram524]
8.1.1.1.8-Singe-tube Sequencing Submission to BRC Protocol [updated 2Aug2016 by ajh22]8.1.1.1.9-Ten Things That Can Kill Your PCR Information Sheet [reviewed by mw16 07072016]
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8.1.1.1.11-Primer Database Search Protocol.pdf SOP [updated 2Sept2016 nrs13]
8.1.1.1.12-rpoB _sigB_ATassignment_User.pdfsigB AT Assignment [MW]
8.1.1.1.13-PCR and Sanger Sequencing Reactions in 96-Well Plates (High-Throughput PCR and Sequencing) SOP [reviewed by mw16 14Sept2015]8.1.1.1.17-pGEM Ladder for Size Determination of PCR Products Recipe [uploaded 17Jan2017 by sjr267]
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8.3.3-Phenol Chloroform Listeria gDNA Extraction_1_28_2015.pdf
8.3.5-Listeria Lysis Methods and Procedures_11_26_13.pdf
8.3.6-PCR product purification using ExoSAP.pdf
8.3.10-Quantification of Purified DNA by NanoDrop.pdf
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8.3.16.4-Example Nextera Library Sample Preparation Spreadsheet Template [reviewed 07Oct2016 by jk2739]
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8.3.18-Preparing Cell Lysates for PCR SOP [updated 2019-10-11 by js2568]
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