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8.1.1.1.9-Ten Things That Can Kill Your PCR Information Sheet [reviewed by mw16 07072016]
8.1.1.1.10-Submitting sequences to GenBank SOP [reviewed by mw16 07072016]
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8.1.1.2.2.2-Gap PCR protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenes.2013 PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and Sequencing SOP [reviewed by mw16 07072016]
8.1.1.2.2.5-Modified SigB PCR Protocol-Taq Gold 10uM primers.pdf (MW)
8.1.1.2.2.11-Prs prs PCR Protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.12-PurM purM PCR Protocol for lineage II Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.13-PurM purM PCR Protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
8.1.1.2.2.14-RibC ribC PCR Protocol for Listeria monocytogenes PCR Protocol [reviewed by mw16 07072016]
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8.1.1.3.4-7 Gene MLST for Salmonella Informational Document [reviewed by mw16 07072016]
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8.1.1.3.7-PCR identification of common Salmonella serogroups SOP [reviewed by mw16 07072016]
8.1.1.3.8-PCR determination of H1 and H2-antigens for Salmonella SOP [reviewed by mw16 07072016]
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8.8.1 Qiagen RNA Midi Prep with RQ1 DNAse treatment Protocol [reviewed by vg93 Spring 2016]
8.8.5-RNA extraction using Qiagen Midi Kit and Turbo DNAse Protocol RNA extraction, clean-up and storage using Turbo DNase for TaqMan.pdfProtocol [reviewed by vg93 Spring 2016]
8.8.10-Taqman- Quantitative Real-Time RT-PCR Protocol Protocol [Updated by vg93 Spring 2016]
8.8.11-RNA Extraction Using TriReagent SOP [reviewed by vg93 4/21/2016]
8.8.12-QuantStudio real time PCRQuantStudio real time PCR protocol (IN PROGRESS).pdfProtocol [reviewed by vg93 Spring 2016]
8.8.13-Sybr Green qRT-PCR Protocol [reviewed by vg93 Spring 2016]
-Make dirty lysates in 100μL of dH2O by picking a colony with a toothpick and scraping into PCR tube
-Microwave dirty lysates for 30s to lyse cells