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8.4.1-Automated Sequence Editing SOP [reviewed by sjr267 03/23/2016]
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8.5-DNA Recombination & Mutagenesis
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8.5.2-Cloning of DNA Fragments.pdf Protocol [reviewed 8Aug2016 mw16]
8.5.3-Cloning PCR products for Sequencing using pCR 2.1.pdf [archive]
8.5.4-Cloning Protocol for Transformation of E. coli DH5aF.pdf [archive]
8.5.5-Electroporation L mono.pdf [archive]
8.5.6-Electroporation of E coli.pdf [archive]
8.5.7-Electroporation of Mycobacterium paratuberculosis.pdf [archive]
8.5.8-General Cloning DNA.pdf [archive]
8.5.9-GUS promoter fusions in Listeria monocytogenes.pdf [keep Protocol [reviewed 08Aug2016 mw16]
8.5.10-Integration of pPL2 derivatives into L. monocytogenes 10403S.pdf [keep]
8.5.11-Listeria Genomic Alterations.pdf [archive]
8.5.12-Overview-Making a mutant in Listeria monocytogenes.pdf [archive]
8.5.13-Part II-Making a mutant in Listeria monocytogenes.pdf [archive]
8.5.14-Part III-Making a mutant in Listeria monocytogenes.pdf [archiveProtocol [reviewed 08Aug2016 mw16]
8.5.15-Part IV-Making a mutant in Listeria monocytogenes.pdf [archive]8.5.16-pMJG2 vector map.jpg Plasmid Map [keepreviewed 08Aug2016 mw16]
8.5.17-pMJG3 vector map.jpg [keep Plasmid Map [reviewed 08Aug2016 mw16]
8.5.18-pMJG4 vector map.jpg [keep]8.5.19-Procedure for cloning with pDH32.pdf [archive Plasmid Map [reviewed 08Aug2016 mw16]
8.5.20-Whole Mutant-Soeing PCR for L. mono SOP [reviewed by bmb29 4/6/2016]
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