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8.1.1.1.1-16S rDNA PCR-Go Taq.pdf [review rachel & Martin, make new 16S protocol]
8.1.1.1.2-16S rDNA PCR-modified for Taq gold.pdf [review Rachel and Martin, make new 16S protocol]
8.1.1.1.3-Basic GoTaq PCR Protocol.pdf [check with current users; keep]
8.1.1.1.4-Colony PCR Protocol.pdf [check with current users - Jeff??; keep]
8.1.1.1.5-Guidelines for Primer Design.pdf Veronica
8.1.1.1.7-Primer_Ordering.pdf nancy
8.1.1.1.8-Sample singe_tube_Sequencing_Submission to BRC.pdf alphina &
8.1.1.1.9-Ten Things That Can Kill Your PCR.pdf keep
8.1.1.1.10-Submitting sequences to GenBank.pdf keep
8.1.1.1.11-Primer Database Search Protocol.pdf nancy
8.1.1.1.12-rpoB_sigB_ATassignment_User.pdf MW
8.1.1.2-Listeria PCR
8.1.1.2.1-Ten Gene MLST for Listeria (keep all)
8.1.1.2.1.1-10 gene MLST scheme.xls
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8.1.1.2.2.1-Fact Sheet on ActA Differentiation PCR and Interpretation of Results.pdf [check with Shelley or sherry, should be superceeded by SOP; archived?]
8.1.1.2.2.2-Gap PCR protocol for Listeria monocytogenes.pdf (keep)
8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenes.2013.pdf (keep)
8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and Sequencing.pdf (keep)
8.1.1.2.2.5-Modified SigB PCR Protocol-Taq Gold 10uM primers.pdf (MW)
8.1.1.2.2.6-Multiplex PCR for inlH screening.pdf (archive)
8.1.1.2.2.7-Nested hly PCR for Listeria monocytogenes.pdf (archive)
8.1.1.2.2.8-Protocol for sequencing actA gene most polymorphic region.pdf (arcive)
8.1.1.2.2.9-Protocol for Sequencing actA gene region with best discrimination.pdf (archive)
8.1.1.2.2.10-Protocol for Sequencing inlA gene region with the best discrimination.pdf (archive)
8.1.1.2.2.11-Prs PCR Protocol for Listeria monocytogenes.pdf (keep)
8.1.1.2.2.12-PurM PCR Protocol for lineage II Listeria monocytogenes.pdf (keep)
8.1.1.2.2.13-PurM PCR Protocol for Listeria monocytogenes.pdf (keep)
8.1.1.2.2.14-RibC PCR Protocol for Listeria monocytogenes.pdf (keep)
8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdf (Yichang and Veronica)
8.1.1.3-Salmonella PCR
8.1.1.3.1-fimA PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf (archive)
8.1.1.3.2-manB PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf (archive)
8.1.1.3.3-mdh PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf (archive)
8.1.1.3.4-7 Gene MLST for Salmonella.pdf (keep)
8.1.1.3.4.1-7 gene MLST for Salmonella- Primers and PCR.xls (check)
8.1.1.3.4.2-Salmonella 7 gene MLST Template.xls (check)
8.1.1.3.5-PCR detection of the presence or absence of antibiotic resistance genes in Salmonella.pdf (archive)
8.1.1.3.6-InvA colony PCR for Salm 4-13-10.pdf (Jeff and Jasna - are we using this for Mars project???; update to reflect current protocol if needed)
8.1.1.3.7-PCR identification of common Salmonella serogroups.pdf (keep)
8.1.1.3.8-PCR determination of H1 and H2-antigens for Salmonella.pdf (keep)
8.1.1.4-PCRs for Other Organisms
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8.1.2.1-Semi-Quantitative Hemolysis Assay for Listeria.pdf [archive]
8.1.3-RFLP
8.1.3.1-Polymerase Chain Reaction- Restriction Fragment Length Polymorphism.pdf [archive]
8.1.4-Southern Blot [archive whole section]
8.1.4.1-Southern Blotting by Electrophoretic Transfer and non radioactive Detection.pdf
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8.1.4.3-SouthernBlot Hybridization.pdf
8.2-DNA Labeling & Modification
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[archive whole section]
8.2.1-3’ end labeling of oligonucleotides.pdf
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8.3.7-PCR Purification Protocol for DNA Sequencing.pdf (Ask MWArchive)
8.3.10-Quantification of Purified DNA by NanoDrop.pdf
8.3.11-Single Stranded DNA preparation from M13.pdf (Archive)
8.3.12-Small-scale phenol-chloroform extraction of chromosomal DNA from Listeria monocytogenes.pdf
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8.3.14-HighThroughput_gDNA_Extraction_Qiagen_1_28_15.pdf
8.3.15-Take3_NucleicAcid_96_well_Quantification_01_20_15.pdf (Ask MWkeep)
8.3.16-DNA Preparation for Whole Genome Sequencing SOP [effective 13Jun2016 ajh22]
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8.4.1-Automated Sequence Editing SOP [reviewed by sjr267 03/23/2016]
8.5-DNA Recombination & Mutagenesis (
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MW to review)
8.5.1-Cloning of DNA Flanking Tn917-LTV3.pdf
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8.7.1-Assay for beta-galactosidase activity of B. subtilis transformed with lacZ reporter plasmid.pdf (mw16 checked07072016)
8.7.2-Beta Galactosidase Assay_general.pdf (mw16 checked07072016)
8.7.3-His tagged sigma factor protein purification protocol.pdf (mw16 checked07072016)
8.8-RNA Prep & Analysis
8.8.1 Qiagen RNA Midi Prep with RQ1 DNAse treatment [reviewed by vg93]
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