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8.8-RNA Prep & Analysis [to be reviewed by Veronica Guariglia]
8.8.1-DNase treatment and phenolchloroform extractions for high purity RNA.pdfQuiagen RNA Midi Prep with RQ1 DNAse treatment.pdf [reviewed by vg93]
8.8.2-Isolation of RNA from Listeria monocytogenes infected mammalian cells.pdf [ARCHIVE]
8.8.3-Preparation of infected tissue culture cells for bacterial RNA collection.pdf [ARCHIVE]
8.8.4-Preperation of Listeria RNA.pdf [ARCHIVE]
8.8.5-RNA extraction, clean-up and storage using Turbo DNase for TaqMan.pdf using Qiagen Midi Kit and Turbo DNAse Protocol [reviewed by vg93]
8.8.6-RNA Prep with RNeasy Midi Kit instructions.pdf [ARCHIVE]
8.8.7-RNeasy kit total RNA isolation from Listeria monocytogenes with on-column DNase treatment.pdf [ARCHIVE]
8.8.8-RT-PCR for the detection of cytokine mRNA.pdf [ARCHIVE]
8.8.9-Synthesis of large amounts of RNA.pdf [ARCHIVE]
8.8.10-Taqman- Quantitative Real-Time RT-PCR Protocol.pdf [Updated by vg93]
8.8.11-RNA Extraction Using TriReagent SOP [reviewed by vg93 4/21/2016]
8.8.12-QuantStudio real time PCR protocol [reviewed by vg93]
8.8.13-Sybr Green qRT-PCR [reviewed by vg93]