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If interested in additional Milk Quality protocols, please contact Nicole Martin at nhw6@cornell.edu

 

Dairy Microbiology:

7.1-2nd floor-16S GreenMaster Procedure.pdf

7.2-16s Identification Using RDP database.pdf

7.3-96 Well Plate Procedure.pdf

7.3.1-96WellPlate Template.xls

7.4-Checking Reports Protocol.pdf

7.5-Colony PCR.pdf

7.7-Endospore Stain - cold.pdf

7.8-Endospore Staining -- Steam Method.pdf

7.9-Final rpoB GreenMaster Protocol.pdf

7.10-Growth Rate L.mono in Milk.pdf

7.11-Highly Heat Resistant Spore Pasteurization SOP.pdf

7.12-Identification of rpoB Sequences using Bioedit.pdf

7.13-Lab Pasteurization.pdf

7.14-PCR Procedure and Touble-shooting Guide for rpoB and 16S.pdf

7.15-Primer Ordering.pdf

7.16-Primer Reconstitution.pdf

7.17-Procedure for Lysis of Bacterial Cells.pdf

7.18-Purified Lysates Procedure.pdf

13-Lab Pasteurization SOP [revised 2020-06-26 rle58]

7.19-Spiral Plating for raw and powdered dairy products SOP [revised 2020-06-26 rle58]

7.25-Determination of silage pH SOP [revised 2020-06-26 rle58]

7.31-Gram Stain SOP [created by sjr167 2020-12-15]

7.32-Direct Microscopic Count of Bacteria and/or Somatic Cells SOP [created by sjr267 2020-12-15]

7.35-Butyric Acid Bacteria Most Probable Number SOP [created by rle58 2024-05-09]


Sporeforming Bacteria:

7.11-Highly Heat Resistant Spore Pasteurization SOP [revised 2020-06-26 rle58]

7.20-Spore Pasteurization SOP [revised 2020-06-26 rle58]

7.21-Spore Media Protocol.pdf

7.28-Specially Thermoresistant Spore Enumeration SOP [created by rle58 2020-06-26]

7.29-Bacterial Cell Extraction from Fluid Milk SOP [created by sjr167 2020-12-15]

7.30-Visualization of Unstained Bacterial Cells using Phase-Contrast Microscopy SOP [created by sjr167 2020-12-15]


Dairy Chemistry:

7.33-Measurement of Total Antioxidant Capacity in Milk SOP [created by sjr267 2023-03-08]

7.33a-TAC Calculation Spreadsheet Document [uploaded 2023-03-14]

7.34-Gerber Method for Butterfat Measurement of Ice Cream SOP [created by sjr267 2023-08-31]


Voluntary Shelf-Life Program:

7.22-VSL Microbiological and Chemical Analysis SOP  [revised by nh376 2020-04-21]

7.27-VSL Sample Shipment Protocol Document [created by asa234 and sim39 15Sept2016]


Outside Work:

7.4-Checking Reports Protocol

Training Record

7.4-Checking Reports Protocol.pdfTrainerJordan Skeens (example)
















































TraineeJordan Skeens (example)
















































Training Date11/02/2020
















































7.11-Highly Heat Resistant Spore Pasteurization SOP.pdfTrainer

















































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7.13-Lab Pasteurization.pdfTrainerRachel
















































TraineeAnthony
















































Training Date6/6/24
















































7.19-Spiral Plating for raw and powdered dairy products.pdfTrainer

















































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7.20-Spore Count SOP.pdfTrainer

















































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7.21-Spore Media Protocol.pdfTrainer

















































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7.22-VSL Microbiological and Chemical AnalysisTrainer

















































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7.26-rpoB Database ManagementTrainer

















































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7.27-VSL Sample Shipment Protocol.pdfTrainer

















































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 16S rDNA protocols can be found under the PCR section of: 8-Molecular Biology Protocols