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1. Move around the microscope until the feature is visible. Ensuring the features are near center of the substrate as possible will prevent bubbles from forming and being moved around the lens.
2. Make sure Center Cursor is on, there should be a dashed blue line on the microscope display. If it is not there, click Center Cursor.
3. Move to one edge of the feature, roughly center, in this case the lowest point of the hole, keeping this in the center of the crosshairs.
4. Move up 1000um on Y by inputting 1000um in the step size box and clicking the up arrow for stage adjustment. Note that in this case picking the Y axis is arbitrary and the X may be used just as easily as a starting point.
5. Realign to the top of the hole, generally 10um increments are sufficient. Record the total distance added or subtracted from the initial 1000um.
6. Move to the new Y center point by inputting half the net distance travelled in Step Size then click the down arrow once e.g. ((1000um-distance correction)/2).
7. Move right on the X axis by current value in Step Size.
8. Align the edge of the hole as with the Y axis. If the center appears to be misaligned with the Y axis, adjustments can be made now to align on this axis.
9. Continue to move side to side and up and down in order to verify that the sample is centered.
10. Record the X and Y coordinates of this center point.
11. For multi-part prints, it is necessary to comment out any instance of FindInterfaceAt  or CenterStage in the _job.gwl and _data.gwl files
    1. In lieu of CenterStage, a new center point can be added manually via the GoToX and GoToY commands, typically in the _job.gwl file.
       1. Formatting is GoToX number where number is the X coordinate of your new center point.

The short video below demonstrates the process for a 25x25 mm substrate with a 1mm center hole. The  The CAD models for this substrate are available for download in STL and STP format, as well as a drawing of the substrate.

The short video below demonstrates the process for a 25x25 mm substrate with a 1mm center hole:

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