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8.1.1.1.5-Guidelines for Primer DesignTrainerJordan Skeens (example)
















































TraineeJordan Skeens (example)
















































Training Date11/02/2020
















































8.1.1.1.5.1-Primer Design ChecklistTrainer

















































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8.1.1.1.7-Primer Ordering and ReconstitutionTrainer

















































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8.1.1.1.9-Ten Things That Can Kill Your PCRTrainer

















































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8.1.1.1.10-Submitting sequences to GenBankTrainer

















































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8.1.1.1.11-Primer Database Search Protocol.pdfTrainer

















































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8.1.1.1.17-pGEM Ladder for Size Determination of PCR ProductsTrainer

















































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8.1.1.1.18-Gel Electrophoresis of PCR ProductTrainerRachel EAlex BSam B














































TraineeZoeSam BMikayla H














































Training Date1/20/21

 

 
















































8.1.1.1.19-PCR Product Purification and Sanger Sequencing SubmissionTrainerRachel EAlex BSam B














































TraineeZoeSam BMikayla H














































Training Date1/21/21

 

 
















































8.1.1.2.2.1-actA PCR Trainer

















































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8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenesTrainer

















































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8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and SequencingTrainer

















































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8.1.1.2.2.5-sigB PCRTrainerAlex BSam B















































TraineeSam BMikayla H















































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8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdfTrainer

















































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8.1.1.3.6-InvA colony PCR for SalmonellaTrainer

















































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8.1.1.3.7-PCR identification of common Salmonella serogroupsTrainer

















































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8.1.1.3.8-PCR determination of H1 and H2-antigens for SalmonellaTrainer

















































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8.1.1.4.1-E. coli 6-gene multiplex PCR.pdfTrainer

















































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8.1.1.4.4-rpoB PCR Protocol TrainerRachel E
















































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Training Date1/20/21
















































8.1.1.4.5-PCR Amplification of Full Length 16s rDNATrainer

















































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8.1.1.4.5.1-PCR Amplification of 16s rDNA Internal RegionTrainer

















































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8.1.1.4.6-ITS PCR for Yeast and MoldTrainer

















































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8.3.10-Quantification of Purified DNA by NanoDrop.pdfTrainer

















































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8.3.13-Genomic_DNA_ Extraction_QiagenTrainer

















































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8.3.14-HighThroughput_gDNA_ Extraction_Qiagen _1_28_15.pdfTrainer

















































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8.3.15-Take3_NucleicAcid_ 96_well_Quantification _01_20_15Trainer

















































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8.3.16-DNA Preparation for Whole Genome SequencingTrainerSophia H
















































TraineeSam B
















































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8.3.16.1-Step-by-step checklist for Gram+ bacteriaTrainerSophia H
















































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8.3.16.2-Step-by-step checklist for Gram- bacteriaTrainer

















































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8.3.16.3-CDC MiSeq Nextera XT Library PreparationTrainer

















































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8.3.16.4-Example Nextera Library Sample Preparation Spreadsheet Trainer

















































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8.3.18-Preparing Cell Lysates for PCRTrainerRachel EAlex BSam B














































TraineeZoeSam BMikayla H














































Training Date

(heat lysis) 

1/20/21

 

 
















































8.4.1-Automated Sequence EditingTrainer

















































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8.4.2-Quality Control and AssemblyTrainer

















































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8.4.3-Short Read Submission (SRA)Trainer

















































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8.4.4-Assembly submission to NCBITrainer

















































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8.4.5-Creating Consensus DNA Sequences using SequencherTrainerAlex BSam B















































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8.4.6-rpoB Allelic Type (AT) AssignmentTrainer

















































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8.5.2-Cloning of DNA FragmentsTrainer

















































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8.5.9-GUS promoter fusions in Listeria monocytogenesTrainer

















































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8.5.10-Integration of pPL2 derivatives into L. monocytogenes 10403STrainer

















































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8.5.16-pMJG2 vector map.jpgTrainer

















































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8.5.17-pMJG3 vector map.jpgTrainer

















































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8.5.18-pMJG4 vector map.jpgTrainer

















































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8.5.20-Whole Mutant-Soeing PCR for L. monoTrainer

















































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8.5.21-P22 Transduction of SalmonellaTrainer

















































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8.5.22-Protocol for Lambda Red Recombinase in SalmonellaTrainer

















































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8.7.1-Assay for beta-galactosidase activity of B. subtilis transformed with lacZ reporter plasmidTrainer

















































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8.7.2-Beta Galactosidase Assay_generalTrainer

















































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8.7.3-His tagged sigma factor protein purification protocolTrainer

















































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8.8.11-RNA Extraction Using TriReagent Trainer

















































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8.8.13-Sybr Green qRT-PCRTrainerAlexa Cohn
















































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Training Date11/19/21
















































8.8.14_RNAext Salmonella.pdfTrainer

















































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