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- Close NanoWrite. This makes sure that used print commands are set to the default values for the next user.
- Only switch off the laser and other printer components if the printer is not in use for a longer period of time.
Multi-DiLL Printing
Multiple
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samples can be printed sequentially without the need to open the printer and switch substrates. This is useful for jobs where a large number of samples are needed, particularly if print times are short. The procedure for setting up a Multi-DiLL print will reference this build folder which is available for download: Sample01_02_03.zip The builds are based on these simple STLs: sampleSTLs.zip.
- Generate STL files as with any standard print job
- Import these files into DeScribe individually and generate a build using your selected solution set. It is important that all files use the same objective, however you may vary between solid and the shell and scaffold solutions.
- Keep the generated files in their own respective folders - organizing these files is very important to the process
- In this case, all files related to Sample1 are in a Sample1 folder
- Combine all sample folders to one master folder
- An example of the file structure is included below:
- Generate a *job.gwl file - this will tell NanoWrite the order of operations and which files to reference
- The example uses 1_2_3_job.gwl and is stored in the master folder. The gwl file can be created in a text editor (by changing .txt to .gwl or similar) and the content should be formatted as follows:
NewStructure NewStructure NewStructure |
|---|
- In this example, we are printing in positions 4, 5, and 6 on the Multi-DiLL holder.
- NewStructure: Tells NanoWrite to begin a new printer
- SamplePosition: Tells NanoWrite which position in the sample holder to print
- Wait: Waits for an integer number of seconds to allow the resin to settle after the lens has approached
- MessageOut: Displays text to indicate a new job has started
- include: Opens the build files from the included path
- You may test your gwl file by opening it in DeScribe to verify the paths are correct. Generate a 3D preview (F5) and ensure there are no errors.
- Note: DeScribe will render all models on top of one another. This is normal behavior, as seen below:
- Copy the folder to the Nanoscribe computer
- Place your substrates in the positions indicated in your gwl file and add resin to them, using an appropriate amount for the sample printed
- Large deposits of resin may drip in the machine, please ensure a minimal amount is applied
- Insert the correct objective and the Multi-DiLL holder as with standard prints
- Select the position of your first print in Nanowrite. By default, the Multi-DiLL is set to position 5, as seen below. To reduce bubble formation in your print, it is best to change this to your first print position. Note that when clicking the new position on the Multi-DiLL graphic in the Nanowrite "Choose sample holder" dialog box, the view is from the bottom of the holder, so positions are flipped, e.g. position 1 is the top right. Click OK and then approach the sample.
- Load the *job.gwl file in your top folder in Nanowrite, in the case of the example load 1_2_3_job.gwl
- Start print job, the total print time scales linearly with the number of samples in the job
Material Specifications for standard printing at CNF
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