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8.1.1.2.2.5-sigB PCR SOP [updated 2020-01-30 by js2568]

8.1.1.2.2.17-Degenerate sigB PCR SOP [created 2020-07-29 by jl3374]

8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdf (Yichang and Veronica)

...

8.1.1.1.5-Guidelines for Primer DesignTrainer                                                  
Trainee                                                  
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8.1.1.1.5.1-Primer Design ChecklistTrainer                                                  
Trainee                                                  
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8.1.1.1.7-Primer Ordering and ReconstitutionTrainer                                                  
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8.1.1.1.9-Ten Things That Can Kill Your PCRTrainer                                                  
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8.1.1.1.10-Submitting sequences to GenBankTrainer                                                  
Trainee                                                  
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8.1.1.1.11-Primer Database Search Protocol.pdfTrainer                                                  
Trainee                                                  
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8.1.1.1.17-pGEM Ladder for Size Determination of PCR ProductsTrainer                                                  
Trainee                                                  
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8.1.1.1.18-Gel Electrophoresis of PCR ProductTrainer                                                  
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8.1.1.1.19-PCR Product Purification and Sanger Sequencing SubmissionTrainer                                                  
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8.1.1.2.2.1-actA PCR Trainer                                                  
Trainee                                                  
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8.1.1.2.2.3-Hly a b gene PCR for Listeria monocytogenesTrainer                                                  
Trainee                                                  
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8.1.1.2.2.4-inlA ORF and Gene Fragment PCR and SequencingTrainer                                                  
Trainee                                                  
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8.1.1.2.2.5-sigB PCRTrainer                                                  
Trainee                                                  
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8.1.1.2.2.16-PCRs For Quadruple sigma factor Mutant.pdfTrainer                                                  
Trainee                                                  
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8.1.1.3.6-InvA colony PCR for SalmonellaTrainer                                                  
Trainee                                                  
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8.1.1.3.7-PCR identification of common Salmonella serogroupsTrainer                                                  
Trainee                                                  
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8.1.1.3.8-PCR determination of H1 and H2-antigens for SalmonellaTrainer                                                  
Trainee                                                  
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8.1.1.4.1-E. coli 6-gene multiplex PCR.pdfTrainer                                                  
Trainee                                                  
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8.1.1.4.4-rpoB PCR Protocol Trainer                                                  
Trainee                                                  
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8.1.1.4.5-PCR Amplification of Full Length 16s rDNATrainer                                                  
Trainee                                                  
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8.1.1.4.5.1-PCR Amplification of 16s rDNA Internal RegionTrainer                                                  
Trainee                                                  
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8.1.1.4.6-ITS PCR for Yeast and MoldTrainer                                                  
Trainee                                                  
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8.3.10-Quantification of Purified DNA by NanoDrop.pdfTrainer                                                  
Trainee                                                  
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8.3.13-Genomic_DNA_ Extraction_QiagenTrainer                                                  
Trainee                                                  
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8.3.14-HighThroughput_gDNA_ Extraction_Qiagen _1_28_15.pdfTrainer                                                  
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8.3.15-Take3_NucleicAcid_ 96_well_Quantification _01_20_15Trainer                                                  
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8.3.16-DNA Preparation for Whole Genome SequencingTrainer                                                  
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8.3.16.1-Step-by-step checklist for Gram+ bacteriaTrainer                                                  
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8.3.16.2-Step-by-step checklist for Gram- bacteriaTrainer                                                  
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8.3.16.3-CDC MiSeq Nextera XT Library PreparationTrainer                                                  
Trainee                                                  
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8.3.16.4-Example Nextera Library Sample Preparation Spreadsheet Trainer                                                  
Trainee                                                  
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8.3.18-Preparing Cell Lysates for PCRTrainer                                                  
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8.4.1-Automated Sequence EditingTrainer                                                  
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8.4.2-Quality Control and AssemblyTrainer                                                  
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8.4.3-Short Read Submission (SRA)Trainer                                                  
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8.4.4-Assembly submission to NCBITrainer                                                  
Trainee                                                  
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8.4.5-Creating Consensus DNA Sequences using SequencherTrainer                                                  
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8.4.6-rpoB Allelic Type (AT) AssignmentTrainer                                                  
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8.5.2-Cloning of DNA FragmentsTrainer                                                  
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8.5.9-GUS promoter fusions in Listeria monocytogenesTrainer                                                  
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8.5.10-Integration of pPL2 derivatives into L. monocytogenes 10403STrainer                                                  
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8.5.16-pMJG2 vector map.jpgTrainer                                                  
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8.5.17-pMJG3 vector map.jpgTrainer                                                  
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8.5.18-pMJG4 vector map.jpgTrainer                                                  
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8.5.20-Whole Mutant-Soeing PCR for L. monoTrainer                                                  
Trainee                                                  
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8.5.21-P22 Transduction of SalmonellaTrainer                                                  
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8.5.22-Protocol for Lambda Red Recombinase in SalmonellaTrainer                                                  
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8.7.1-Assay for beta-galactosidase activity of B. subtilis transformed with lacZ reporter plasmidTrainer                                                  
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8.7.2-Beta Galactosidase Assay_generalTrainer                                                  
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8.7.3-His tagged sigma factor protein purification protocolTrainer                                                  
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8.8.11-RNA Extraction Using TriReagent Trainer                                                  
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8.8.13-Sybr Green qRT-PCRTrainer                                                  
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8.8.14_RNAext Salmonella.pdfTrainer                                                  
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8.1.1.2.2.16